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Running BLAST bioinformatics binary

Hi

I currently have some executable (the NCI-BLAST algorithm...makeblastdb) that I want to run from the python script. I am wondering why is that after I set the permission with "chmod +x "path to/makeblastdb".....it says "no such file or direcotry" Or is there any other way to run the binary ?

The chmod command certainly should work. Are you sure you're using the exact pathname for makeblastdb? For example, might you be using a relative path while you're not in the right directory?

Thanks for prompt reply. Finally got it to work. Because I used a 32 bit binary. In case anyone needs to do the same, you need a 64 bit binary ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/2.2.24/ncbi-blast-2.2.24+-x64.linux.tar.gz worked for me

Ah, that would explain it. Thanks for posting the explanation!